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(2017.9.21)Single cell analysis of antigen-specific T cell response against Mycobacterium tuberculosis
发布日期:2017-09-20浏览次数:字号:[ ]

   

Dear all:


 

北京大学分子医学研究所
血管生物学实验室
Seminar Announcement

 

目:Single cell analysis of antigen-specific T cell response against Mycobacterium tuberculosis


 

报告人:Huang Huang, Ph.D.IMM优秀毕业生)

                Research Associate,

                Institute for Immunity, Transplantation, and Infection, Stanford University, USA


 

间:2017921日(星期四)下午4:00-5:30


 

点:北京大学英杰交流中心321会议室


 

Abstract: It is estimated that one-third of the worlds population is latently infected with M. tuberculosis (Mtb). While only 10% of the infected individuals develop progressive disease after exposure to Mtb. The mechanisms underlying protection against infection and/or disease are not well understood and there are no immunologic or other biomarkers known to predict the efficacy of vaccine candidates without the long and costly progression to Phase IIB clinical trials. In order to study the T cell response against Mtb, here we are using single-cell T cell receptor (TCR) sequencing to study the TCR repertoire of Mtb-reactive T cells and demonstrated in vivo clonal expansion, public TCR motifs and Th1* like phenotypes of Mtb-reactive T cells. We also developed and optimized a method to capture both TCR and transcriptome from the same single T cell with more than 80% successful rate in sorted single T cell. It thus allows functional study of T cell clones (cells with identical TCRs) directly from blood or other tissues without in vitro expansion. Using this method, we have identified conventional and unconventional (MAIT/NKT) cells reactive to Mtb. Transcriptome analysis of clonal expanded cells identified a feature of clonal senescence among conventional T cells but not among unconventional T cells.

 

联系人:北京大学分子医学研究所   罗金才(Tel.6275-0922) 

 




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